![]() ![]() Being one of the most widely studied growth inhibitors for E. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: This project is partially funded with Life Technologies, and there are two co-authors from Life Technologies, but this does not alter our adherence to all the PLOS ONE policies on sharing data and materials.Īcetate, either lignocellulosic-derived or as a fermentative byproduct, can pose a major problem in microbial bioprocesses, especially in the presence of excess glucose. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: This project is supported by Ministry of Education, Singapore (RG27/11) and Life Technologies CRC program. Received: JAccepted: SeptemPublished: October 4, 2013Ĭopyright: © 2013 Chong et al. PLoS ONE 8(10):Įditor: Mark Isalan, Center for Genomic Regulation, Spain coli acetate sensitivity.Ĭitation: Chong H, Yeow J, Wang I, Song H, Jiang R (2013) Improving Acetate Tolerance of Escherichia coli by Rewiring Its Global Regulator cAMP Receptor Protein (CRP). Eight genes were chosen for overexpression and the overexpression of uxaB was found to lead to E. Real-time PCR analysis via OpenArray ® system revealed that over 400 CRP-regulated genes were differentially expressed in A2 with or without acetate stress, including those involved in the TCA cycle, phosphotransferase system, etc. Mutant A2 (D138Y) was isolated and its growth rate in 15 g/L sodium acetate was found to be 0.083 h -1, much higher than that of the control (0.016 h -1). Error-prone PCR method was employed to modify crp and the mutagenesis libraries (~3×10 6) were subjected to M9 minimal medium supplemented with 5–10 g/L sodium acetate for selection. coli was much improved by rewiring its global regulator cAMP receptor protein (CRP), which is reported to regulate 444 genes. In this work, the acetate tolerance of E. coli strains with enhanced acetate tolerance would be valuable for these bioprocesses. coli fermentation due to its inhibitory effect on cell growth, thereby limiting high-density cell culture and recombinant protein production. Among the nine albums he recorded between 19 was 'No Way!' - an LP that is much-coveted by crate diggers, and now reissued by VMP.The presence of acetate exceeding 5 g/L is a major concern during E. Though not as well-known as Jimmy Smith and Stanley Turrentine, a bespectacled guitar player named Boogaloo Joe Jones also made his mark as an exponent of soul jazz. As some jazz musicians were pondering how they could compete with the relentless rise of pop, Motown and rock ’n’ roll acts, others - the avant-garde players - were seeking to take the music into another dimension altogether, where freedom of artistic expression overruled all commercial considerations. Not only was it battling to keep its existing audience, it was also a genre seemingly at war with itself. "In the early 1960s, jazz was facing an existential crisis. It's here on AAA 180g black audiophile vinyl, plated at RTI and pressed at GZ with lacquers cut from the original master tapes by Ryan Smith of Sterling Sound. No Way! is the fifth album from master jazz guitarist Boogaloo Joe Jones, and the VMP Classics Record of the Month for April 2022. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |